Regulatory
mLacI

Part:BBa_K1639011:Design

Designed by: Nurgeldi Bazarov   Group: iGEM15_ATOMS-Turkiye   (2015-09-15)


mammalian LacI with miR-223 and miR-21 binding sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1201
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 787
    Illegal BsaI.rc site found at 1165


Design Notes

We suggest also to view similar part we submitted-BBa_K1639010. It contains mLacI with miR binding sites but have restriction enzyme cut sites to remove miR binding site as needed.


Source

This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators in nucleus.

References

Xie Z., Wroblewska L., Prochazka L., Weiss R. ve Benenson Y. 2011. "Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells". Science, 333(6047), 1307-1311.